Coding

Part:BBa_K1779100:Design

Designed by: Guang Zhang   Group: iGEM15_CHINA_CD_UESTC   (2015-09-04)


Magnetosome mamCD operon and mms6 operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2533
    Illegal NheI site found at 3674
    Illegal NheI site found at 4507
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 335
    Illegal BamHI site found at 5584
    Illegal XhoI site found at 602
    Illegal XhoI site found at 1484
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3226
    Illegal NgoMIV site found at 4271
    Illegal NgoMIV site found at 6030
    Illegal NgoMIV site found at 6120
    Illegal AgeI site found at 5203
    Illegal AgeI site found at 5455
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 639
    Illegal BsaI site found at 4237
    Illegal BsaI site found at 4707
    Illegal BsaI site found at 4974
    Illegal BsaI site found at 6095
    Illegal BsaI.rc site found at 2756
    Illegal SapI site found at 3094
    Illegal SapI site found at 4656
    Illegal SapI site found at 5809


Design Notes

First we found the two operon's sequences from NCBI. Then we designed a pair of primers to amplify the DNA fragment of the two operons. When designing the primers, we designed a 20bp overlap and then we used the overlap to join them together with fusion PCR.

Source

Magnetospirillum gryphiswaldense MSR-1 v2, complete genome

References

Ana Carolina V. Araujo; Fernanda Abreu; Karen Tavares Silva; Dennis A. Bazylinski; Ulysses Lins. Magnetotactic Bacteria as Potential Sources of Bioproducts.Mar. Drugs 2015,13,389-430